Delvotest® T is a broad spectrum microbial inhibition test for the detection of antibacterial substances in milk. It is compliant with Maximum Residue Limits and has a higher sensitivity for Tetracyclines. It can be applied with cows’, sheep and goats’ milk. This video shows how to use the test. Wash your hands and work on a clean surface. Ensure the milk sample is fresh and representative. We advise to also use a reference sample from the same type of milk. Preheat the incubator to 64°C. Place the incubator in a draught free area, do not place near open windows. Open the ampoules by punching a small hole in the aluminum foil and label each ampoule. Homogenize the milk sample prior to pipetting. Use a clean pipette for every sample. Dip the tip in the milk and dispense it into the ampoule. Do not touch the agar with the pipette. Check the temperature of the incubator (64°C +/- 2°C) and insert the ampoules into the incubator. After 3 hours and 15 minutes read the color visible at the lower end of the solid agar. Yellow is negative which means the milk is free from antibacterial substances. Purple means that the milk does contain antibacterial substances. Alternatively incubate the ampoules in a water bath at specified temperature avoiding condensed water dripping on the test. Store Delvotest® T after use at the specified conditions of 4-8 °C dark upright in the original packaging. The best test sensitivity is reached when using the control time (CT).
To measure at control time, use one test with negative control milk and stop the incubation of the test when the test with the control milk has turned yellow then read the result of the sample.